u251 (the human malignant glioma cell lines, no. cl-0237)  (Procell Inc)

Journal: Materials Today Bio

Article Title: Doxorubicin-loaded PEGylated liposome modified with ANGPT2-specific peptide for integrative glioma-targeted imaging and therapy

doi: 10.1016/j.mtbio.2025.101455

Figure Lengend Snippet: High ANGPT2 expression correlates with GBM progression and poorer prognosis. (A) ANGPT2 mRNA expression in different malignant tumors ( http://gepia.cancer-pku.cn/ ). (B) Comparison of ANGPT2 mRNA expression between normal brain and GBM tissues from TCGA datasets. (C) ANGPT2 mRNA expression in brain tumor at different stages ( http://www.cgga.org.cn/ ), # p < 0.001. (D) ANGPT2 mRNA expression in brain tumor at WHO stage IV in individuals of different gender and age ( http://www.cgga.org.cn/ ). (E) Kaplan-Meier curves estimating overall survival and (F) disease-free survival ( https://smuonco.shinyapps.io/PanCanSurvPlot ) in patients of ANGPT2 mRNA in GBM. Log-rank test, p < 0.001. (G) The observation of ANGPT2 protein expression in different stages of brain tumor tissues. (H) Representative images of ANGPT2 protein expression (a, c, e, and g, magnification 5 × ; b, d, f, and h, magnification 200 × , bar = 50 μm). (I) Positive rate of ANGPT2 in glioma in different stages of brain tumor tissues. (J, K) ANGPT2 expression was analyzed using IB in HA, T98G, U251, and U87-MG cells. ANGPT2 expression was quantified (ANGPT2/β-actin). Normalized ANGPT2 in HA cells was set at 1.0. ∗ p < 0.05, ∗∗ p < 0.01 ( n = 3). (L, M) ANGPT2 levels in membrane proteins were analyzed using IB in T98G, U251, and U87-MG cells. The level of ANGPT2 was quantified (ANGPT2/Na + -K + ATPase). IB, immunoblotting; HA, human astrocyte cell.

Article Snippet: U251 (The human malignant glioma cell lines, no. CL-0237) (Procell, China), HA (The human astrocyte cell lines, no.1800) (ScienCell Research Laboratories, San Diego, CA, USA), U87-MG (The human malignant glioma cell lines, no. CL-0238) (Procell, China), U87-MG-luc (The human astrocyte tumors cell line, no. WZ0028) (Fenghui Bio, Changsha, China) and bEND.3 (The mouse brain-derived endothelial cells.3, no. TCM-C715) (Hycyte Biotechnology, Suzhou, China) were cultured in DMEM medium with 10 % fetal bovine serum.

Techniques: Expressing, Comparison, Membrane, Western Blot

Journal: Plants

Article Title: Characterization and Toxicity of Hypoxoside Capped Silver Nanoparticles

doi: 10.3390/plants11081037

Figure Lengend Snippet: Cytotoxicity of HE-AgNPs ( A ) and HP-AgNPs ( B ) on the U251, U87, and HaCaT cell lines as obtained from MTT assays.

Article Snippet: The World Health Organization grade IV human malignant glioma cell lines U251 (ECACC 09063001, European Collection of Authenticated Cell Cultures) and U87 (ATCC HTB-14, American Type Culture Collection) were generously donated by the Prince Laboratory, Faculty of Health Sciences, University of Cape Town, Cape Town (Omoruyi et al., 2020), while the human keratinocyte cell line HaCaT (used as a normal non-cancerous cell line) (Boukamp et al., 1988) was a generous donation by the Mintek Laboratory, Department of Biotechnology, University of the Western Cape.

Techniques:

Journal: Plants

Article Title: Characterization and Toxicity of Hypoxoside Capped Silver Nanoparticles

doi: 10.3390/plants11081037

Figure Lengend Snippet: IC 50 values and selectivity index (SI) of AgNPs.

Article Snippet: The World Health Organization grade IV human malignant glioma cell lines U251 (ECACC 09063001, European Collection of Authenticated Cell Cultures) and U87 (ATCC HTB-14, American Type Culture Collection) were generously donated by the Prince Laboratory, Faculty of Health Sciences, University of Cape Town, Cape Town (Omoruyi et al., 2020), while the human keratinocyte cell line HaCaT (used as a normal non-cancerous cell line) (Boukamp et al., 1988) was a generous donation by the Mintek Laboratory, Department of Biotechnology, University of the Western Cape.

Techniques:

Journal: Journal of Korean Medical Science

Article Title: Silencing of Long Non-Coding RNA MALAT1 Promotes Apoptosis of Glioma Cells

doi: 10.3346/jkms.2016.31.5.688

Figure Lengend Snippet: MALAT1 was highly expressed in glioma patients and cell lines. ( A ) 37 glioma tissue samples and adjacent normal brain samples were selected, and then qRT-PCR assay was employed to detect the relative expression level of MALAT1 in glioma patients. The adjacent normal brain tissues (paracancer group) were used as the negative control. ( B ) Human malignant glioma cell lines U87 and U251 cells were cultured, and then qRT-PCR assay was employed to detect the relative expression level of MALAT1 in both cells. The normal glia cell line NHA was used as the negative control. ( C ) U87 and U251 cells were transfected with siRNA targeting MALAT1, QRT-PCR assay was employed to detect the endogenous expression of MALAT1 to confirm the knockdown efficiency. The glioma cells transfected with Scramble was used as the negative control. * P < 0.05 vs. the control; † P < 0.01 vs. the control.

Article Snippet: Normal glia cell line NHA and the human malignant glioma cell lines U87 and U251 were acquired from the Beijing Zhongyuan Company (Beijing, China).

Techniques: Quantitative RT-PCR, Expressing, Negative Control, Cell Culture, Transfection, Knockdown, Control

Journal: Journal of Korean Medical Science

Article Title: Silencing of Long Non-Coding RNA MALAT1 Promotes Apoptosis of Glioma Cells

doi: 10.3346/jkms.2016.31.5.688

Figure Lengend Snippet: Knockdown of MALAT1 decreased the growth of glioma cells. ( A, B ) U87 and U251 cells were seeded into 96-well plates and cultured for 0 hours, 24 hours, 48 hours, 72 hours and 96 hours respectively, MTT assay was employed to detect the effect of MALAT1 knockdown on cell growth at different time points in both cells. The glioma cells transfected with Scramble was used as the negative control. * P < 0.05 vs. the control. ( C, D ) U87 and U251 cells in serum-free medium were placed into the upper transwell chamber, the invasion assays in vitro were carried out to detect the effect of MALAT1 knockdown on cell invasion after 24 hours of incubation. The glioma cells transfected with Scramble was used as the negative control. * P < 0.05 vs. the control.

Article Snippet: Normal glia cell line NHA and the human malignant glioma cell lines U87 and U251 were acquired from the Beijing Zhongyuan Company (Beijing, China).

Techniques: Knockdown, Cell Culture, MTT Assay, Transfection, Negative Control, Control, In Vitro, Incubation

Journal: Journal of Korean Medical Science

Article Title: Silencing of Long Non-Coding RNA MALAT1 Promotes Apoptosis of Glioma Cells

doi: 10.3346/jkms.2016.31.5.688

Figure Lengend Snippet: Knockdown of MALAT1 increased the apoptosis rate of glioma cells. ( A, B ) U87 and U251 cells were seeded in 6 well plates and grow to a confluency of 70%, then both cells were transfected with si-MALAT1 or Scramble respectively. Annexin V-FITC/PI double staining assay was employed to detect the effect of MALAT1 knockdown on the apoptosis rate in U87 ( A ) and U251 ( B ) cells. The glioma cells transfected with Scramble was used as the negative control. FL2-H, FL2-Height.

Article Snippet: Normal glia cell line NHA and the human malignant glioma cell lines U87 and U251 were acquired from the Beijing Zhongyuan Company (Beijing, China).

Techniques: Knockdown, Transfection, Double Staining, Negative Control

Journal: Journal of Korean Medical Science

Article Title: Silencing of Long Non-Coding RNA MALAT1 Promotes Apoptosis of Glioma Cells

doi: 10.3346/jkms.2016.31.5.688

Figure Lengend Snippet: CCND1 and MYC was down-regulated with MALAT1 knockdown. U87 and U251 cells were seeded in 60 mm plates and grow to a confluency of 70%, then both cells were transfected with si-MALAT1 or Scramble respectively. QRT-PCR and western blot analysis were employed to verify the change of expression levels of CCND1 and MYC in response to MALAT1 knockdown in U87 cells ( A, B ) and U251 cells ( C, D ). The cells transfected with Scramble was used as the negative control. * P < 0.05 vs. the control; † P < 0.01 vs. the control.

Article Snippet: Normal glia cell line NHA and the human malignant glioma cell lines U87 and U251 were acquired from the Beijing Zhongyuan Company (Beijing, China).

Techniques: Knockdown, Transfection, Quantitative RT-PCR, Western Blot, Expressing, Negative Control, Control